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Tuesday, May 19, 2020 | History

6 edition of Characterization and Physiological Importance of a Novel Cyclic-B2-Kinase in Xenopus Laevis found in the catalog.

Characterization and Physiological Importance of a Novel Cyclic-B2-Kinase in Xenopus Laevis

Rita Derua

Characterization and Physiological Importance of a Novel Cyclic-B2-Kinase in Xenopus Laevis

by Rita Derua

  • 94 Want to read
  • 13 Currently reading

Published by Coronet Books Inc .
Written in English

    Subjects:
  • Proteins,
  • Enzymology,
  • Science/Mathematics

  • Edition Notes

    SeriesActa Biomedica Lovaniensia, 116
    The Physical Object
    FormatPaperback
    Number of Pages117
    ID Numbers
    Open LibraryOL12846915M
    ISBN 109061867045
    ISBN 109789061867043
    OCLC/WorldCa36978169

    A novel gene expressed in the early evening in photoreceptor cells was isolated and named nocturnin for night factor. This article outlines the steps we took to study a protein of unknown function, particularly highlighting the analyses one can perform when little more than the primary sequence of Cited by: Although all neural crest cells undergo EMT, the timing of delamination occurs at different stages in different organisms: in Xenopus laevis embryos there is a massive delamination that occurs when the neural plate is not entirely fused, whereas delamination in MeSH: D

      Introduction. Osmolality in the brain is an important physiological parameter that is under stringent homeostatic control. Activity-dependent fluxes of osmolytes and the volume of the extracellular space (ECS) are disproportionately regulated by astrocytes, which take up neuroactive substances (glutamate, K +, protons) and redistribute water among neuronal, glial, vascular, and ECS Cited by: Abstract. The embryo of the African clawed frog Xenopus laevis plays a central role in the field of cell and developmental biology. One of the strengths of Xenopus as model system lies in the high degree of conservation between amphibians and mammals in the molecular mechanisms controlling tissue patterning and by: 3.

      Cloning and characterization of zebrafish cyclin Dx. Mammals possess three members of the cyclin D family: cyclin D1, D2 and D3 13,14,43,In Xenopus, cyclin D1 Cited by: 2. Characterization and Physiological Importance of a Novel Cell Cycle Regulated Protein Kinase inXenopus laevisOocytes That Phosphorylates Cyclin B2. The PR55 and PR65 Subunits of Protein Phosphatase 2A from Xenopus laevis. Molecular Cloning and Developmental Regulation of Expression Molecular Biology of the Cell is published by the.


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Characterization and Physiological Importance of a Novel Cyclic-B2-Kinase in Xenopus Laevis by Rita Derua Download PDF EPUB FB2

Phosphopeptide analysis identified Ser53 as the majorin vitrophosphorylation site for cyk in cyclin B2. Using a synthetic peptide derived from cyclin B2 encompassing Ser53 (cyktide) as a substrate, cyk was shown to be activated during progesterone-induced maturation, with Cited by: 8. Get this from a library.

Characterization and physiological importance of a novel cyclin-B2-kinase in Xenopus laevis. [Rita Derua]. Characterization and physiological importance of a novel cell cycle regulated protein kinase in Xenopus laevis oocytes that phosphorylates cyclin by: 8.

Cloning and Functional Characterization of a Novel Aquaporin fromXenopus laevis Oocytes Article in Journal of Biological Chemistry (43) November with 13. As we move further into the 21st century, the advancement and applications of novel technologies in Xenopus means that this well established and powerful model organism will continue to prove an attractive model for human disease mechanisms for many decades in the by: 1.

Xenopus laevis is the most widely used model amphibian species in laboratories, yet there is almost no experimental evidence to guide best practice for captive housing. Enrichment is an important. Here, we present the functional and physiological characterization of the INT1 protein, analyses of the tissue-specific expression of the INT1 gene, and analyses of phenotypic differences observed between wild-type plants and mutant lines carrying the int and int alleles.

Introduction. Inthe editors Peter Nieuwkoop and Job Faber published the Normal Table of Xenopus laevis (Daudin) (hereafter referred to as Normal Table), a work whose practical importance to developmental biology cannot be overstated.

Part of that volume, perhaps the most frequently used part, is a set of drawings by J. Prijs that were based on pencil drawings by Job Cited by: 6.

In this respect, the African clawed frog, Xenopus laevis, has a long history in scientific research, as a major non-mammalian laboratory animal model in vertebrate physiology, biochemistry and.

Plants have multiple potassium (K+) uptake and efflux mechanisms that are expressed throughout plant tissues to fulfill different physiological functions. Several different classes of K+ channels and carriers have been identified at the molecular level in plants.

K+ transporters of the HKT1 superfamily have been cloned from wheat (Triticum aestivum), Arabidopsis, and Eucalyptus. The third subfamily of PKC, atypical PKC (aPKC), exhibits phospholipid-dependent kinase activity.

The physiological importance of cPKC and nPKC is clear because of their properties as phorbol ester/diacylglycerol receptors, but that of aPKC remains to be clarified. PKC isoforms consist of conserved domains that are separated by variable sequences.

The α3β4 nAChRs are implicated in pain sensation in the PNS and addiction to nicotine in the CNS. We identified an α-4/6-conotoxin (CTx) TxID from Conus textile. The new toxin consists of 15 amino acid residues with two disulfide bonds.

TxID was synthesized using solid phase methods, and the synthetic peptide was functionally tested on nAChRs heterologously expressed in Xenopus laevis by: important model system.

KEY WORDS: Xenopus laevis, Embryonic development, Stage series, Craniofacial patterning Introduction Inthe editors Peter Nieuwkoop and Job Faber published the Normal Table of Xenopus laevis (Daudin) (hereafter referred to as Normal Table), a work whose practical importance to developmental biology cannot be Cited by: 6.

We have approached this problem by studying the tRNA genes of the frog Xenopus laevis, and in particular the organization of the fold reiterated genes coding for the initiator methionyl-tRNA (2). Earlier work has shown that these genes are intermingled with long spacer sequences to form tandem repeating units of DNA (3,4).

Models of Seizures and Epilepsy, Second Edition, is a valuable, practical reference for investigators who are searching for the most appropriate laboratory models to address key questions in the field. The book also provides an important background for physicians, fellows, and students, offering insight into the potential for advances in epilepsy research as well as R&D drug development.

Cyclic nucleotide-gated (cng) non-selective cation channels have been cloned from a number of animal systems. These channels are characterized by direct gating upon cAMP or cGMP binding to the intracellular portion of the channel protein, which leads to an increase in channel conductance.

Animal cng channels are involved in signal transduction systems; they translate Cited by: Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay. GLUT Characterization Using Frog Xenopus laevis Oocytes.

Ensembl ENSG ENSMUSG UniProt Q9ULW0 A2APB8 RefSeq (mRNA) NM_ NM_ NM_ NM_ NM_ NM_ RefSeq (protein) NP_ NP_ NP_ NP_ NP_ NP_ Location (UCSC) Chr – Mb Chr 2: – Mb PubMed search Aliases: TPX2, C20orf1, C20orf2, DIL-2, DIL2, FLS.

three novel Xenopus laevis GLPlike peptides with - 70 % amino acid identity to human GLP-1 were found to be similar to that of human GLP-I.

Furthermore residues at positions 12 and 23 of the Xenopus peptides, corresponding to positions 19 and 29 of mammalian GLP-1 were identified as critical to peptide activity. Study of GIPIGLP-1Author: Malathy Satkunarajah. A simple technique is described for fractionating cells of Xenopus laevis using Triton X and citric acid.

Cells are treated for 1 min with a hypotonic buffer containing % Triton X and. Uozumi, N. et al. The Arabidopsis HKT1 gene homolog mediates inward Na + currents in Xenopus laevis oocytes and Na + uptake in Saccharomyces cerevisiae. Cited by: Models of Seizures and Epilepsy, Second Edition, is a valuable, practical reference for investigators who are searching for the most appropriate laboratory models to address key questions in the book also provides an important background for physicians, fellows, and students, offering insight into the potential for advances in epilepsy research as well as R&D drug development.

Heterologous expression and electrophysiological characterization. The heterologous expression of the original AaSlif transcript (GenBank accession #: KM) in Xenopus laevis Cited by: